Effects of Gas Atmospheres and Glucose on the Development of Bovine Embryos Derived from in Vitro Fertilization
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منابع مشابه
Highly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System
Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...
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Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...
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Purpose: The aim of this study was to evaluate the effect of different modifications of sequential synthetic oviductal_fluid (SOF) culture system on developmental competence of in vitro matured/fertilized cattle embryos. Materials and Methods: Bovine oocytes were matured and fertilized in vitro and then presumptive zygotes were randomly cultured for up to 9 days in different modifications of SO...
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Background: In recent years, a combination of hormones and agents in assisted reproductive technology (ART) cycles as a means of stimulating the ovary and developing more follicles. With respect to the positive effects of Estradiol Valerate on reproductive tissues and the impact on native genes of the egg that transferred to the embryo after fertilization, it seems that this agent can also effe...
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The study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gFF) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in Black Bengal goats. Follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. Media were prepared using TCM-199 supplemented wi...
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